Silver nanoparticles of size ranging from 1 ∼ 100 nm are petite metallic colloidal particles, with its applications in diagnostics, biomarkers, imaging, cell labeling and drug delivery. Fungus-mediated synthesis of silver nanoparticles is an ecofriendly and green process with a comparatively simpler downstream processing. In the present study, the ability of macrofungi and pine stand soil fungi was evaluated for their ability to synthesize both extracellular as well as intracellular silver nanoparticles. When the macro and microfungi were challenged with 1 mM silver nitrate, colour change of the cell free filtrates indicated the formation of silver nanoparticles. The presence of silver nanoparticles was confirmed by Surface Plasmon Resonance absorption band in visible wavelength visualized every 24h upto 72h.Silver nanoparticles are known to possess a sharp peak in a range of 400-450 nm and peaks observed at 457nm, 403nm and 414nm by mushroom support their synthesis in comparison to that of 349nm by soil fungi. Transmission Electron Microscopic analysis of the silver nanoparticles revealed the nanorange, dimensions and structural conformation ofbio synthesized nanoparticles. Synergistic study of the synthesized nanoparticles revealed a significant antibacterial activity against four pathogens viz. MTCC 730 (Escherichia coli), MTCC 1925 (Streptococcus pyogenes), MTCC 96 (Staphylococcus aureus) and MTCC 430 (Bacillus cereus).Additionally, the silver nanoparticles inhibited the growth of the yeast pathogen MTCC 183 (Candida albicans) which showed synergistic enhancement in activity along with flucanazole. The fungal samples were analyzed for phytochemical constituents who led to reduction of silver nitrate into nanoparticles. The results obtained indicated that the experimental voucher fungus are more competent than soil fungi in synthesizing silver nanoparticles and can be used a potent natural antibacterial source for various pharmaceutical and textile applications.