The present study was aimed to elucidate the salt tolerant, salt inducible and salt sensitive protein of the Vigna unguiculata by Sodium Deodyl Sulphate – Poly Acrylamide Gel Electrophoresis. Seedlings of Vigna unguiculata exposed to different environmental conditions exhibited a plethora of physio-chemical responses. The seedlings treated with various concentrations of NaCl at third day showed maximum of 85 bands, with nine active regions and their MW-Rf values ranged from 0.012 to 0.891. The seedlings treated with same experimental set up at fifth day showed maximum number of 63 bands with eight active regions and their MW-Rf values ranged from 0.108 to 0.891. On 5th day seedlings showed the isoperoxidase expression with various sizes of bands. The soluble protein showed a gradual increase during the initial stage and after fifth day there was gradual decrease in their content.