Sol-gel method opens new possibilities for synthesis of anomaterials with application in biotechnology and medicine as matrices for immobilization of cells, enzymes and other biomolecules. The main purpose of the present work is to study of the sol-gel synthesis and the structure of silica hybrid nanomaterials containing different quantities of organic component. FT-IR spectra show that in hybrids synthesized by ETMS or MTES strong chemical bonds are observed. The average size of nanoparticles on the sample surface is about 20 - 50 nm and formation of self-organized structures is observed.