Aims: To develop and subsequently validate a simple reversed-phase high-performance liquid chromatography (HPLC) method for estimation of Cefpirome sulphate (CPS) present in pharmaceutical dosage forms. Materials and Methods: The proposed RP-HPLC method utilizes a LiChroCART-Lichrosphere100, C18 RP column (250 mm×4 mm×5 μm) in an isocratic separation mode with mobile phase consisting of methanol and 5mM Tetra butyl ammonium hydrogen sulphate in the proportion of 50:50 % (v/v), at a flow rate 1ml/min and the effluent was monitored at 270 nm using Doxophylline (DXP) as a internal standard. Results: The retention time of CPS and DXP were 2.26 min. and 3.78 minutes respectively. The described method was linear over a range of 0.5-300 μg/ml. The percentage recovery was 101.163. F-test and t-test at 95% confidence level were used to check the intermediate precision data obtained under different experimental setups; the calculated value was found to be less than critical value. Conclusions : The LC method described here is a very simple, sensitive, and accurate procedure for estimation of CPS. The developed and validated LC method is specific, accurate, robust and precise analysis of CPS in pure and its formulations.