Objective: To investigate the conversion potential of alginate encapsulated nodes of Glycyrrhiza glabra with phyto-chemical evaluation of root extract of field transferred plants. Methods: The excised axenic nodal segments were encapsulated in alginate matrix planted on Murashige and Skoog (1962) medium with different supplementation and formulations of PGRs. The two year old field transferred plants were evaluated for phyto-compounds analysis using GC-MS technique. Results: Varied responses were observed during the study, maximum conversion 95.83% ± 2.40% was obtained in these encapsulates when planted on MS medium containing 2.5 μM Kinetin and 0.5 μM α-Naphthalene acetic acid, which eventually developed into complete plantlets in a single step. Further, GC-MS analysis showed the presence of different phyto-compounds in the methanolic root extracts of in vitro converted plants. The results obtained revealed the presence of about 47 phyto-compounds along with various potential bioactive compounds useful for industrial and pharmaceutical purposes. Conclusions: This study investigates high frequency regeneration and conversion of Glycyrrhiza glabra in a single step in short time. Also, the in vitro raised plants are analysed for bioactive compounds after field transfer, which shows the presence of numerous compounds useful for commercial and pharmacological purposes.