Biometric based authentication systems provide solutions to the problems in high security which remain with conventionalsecurity systems. In a biometric verification system, human’s biological parameters (such as voice, finger print,palm print or hand geometry, face, iris etc.) are used to verify the authenticity of a person. These parameters are good to beused as biometric parameters but do not provide the guarantee that the person is present and alive. As voice can be copied,finger print can be picked from glass on synthetic skin and in face recognition system due to genetic factors identical twinsor father-son may have the same facial appearance. ECG does not have these problems. It can not be recorded without theknowledge of the person and ECG of every person is unique even identical twins have different ECG. In this paper an ECGbasedbiometrics verification system which was developed using Laboratory Virtual Instruments Engineering Workbench(LabVIEW) version 7.1 is discussed. Experiments were conducted on the database stored in the laboratory of 20 individualshaving 10 samples each and the results revealed a false rejection rate (FRR) of 3% and false acceptance rate (FAR) of 3.21%.