Traditional agricultural systems are challenged by globally declining resources resulting from climate change and growing population. Alternative agricultural practices such as aquaponics (includes crop plant and aquatic species) and hydroponics (includes crop plant only) have the potential to generate high yield per unit area using limited land, water, and no soil. A soilless agricultural study was conducted at the Georgia Southern University, Statesboro, GA, USA from August to November, 2015. The growth, yield, quality, and nutrition of basil (Ocimum basilicum L.) cultivar Aroma 2, were compared between aquaponic and hydroponic systems using crayfish (Procambarus spp.) as the aquatic species. Non-circulating floating raft systems were designed using 95 L polyethylene tanks. Equal amounts of start-up fertilizer dose were applied to both systems. The objective was to understand how the additional nutritional dynamics associated with crayfish influence the basil crop. Both fresh and dry basil plant weights were collected after harvest, followed by leaf nutrient analysis. Leaf chlorophyll content, water pH, nitrogen and temperature were measured periodically. Aquaponic basil (AqB) showed 14%, 56%, and 65% more height, fresh weight, and dry weight, respectively, compared to hydroponic basil (HyB). It is logical to assume that crayfish waste (excreta and unconsumed feed) has supplied the additional nutrients to AqB, resulting in greater growth and yield. The chlorophyll content (plant quality) or leaf nutrients, however, did not differ between AqB and HyB. Further research is needed to investigate aquaponic crayfish yield, overall nutritional dynamics, cost-benefit ratio, and other plant characteristics under soilless systems.