The purpose of this study is to investigate the effects of ingesting either a high glycaemic index (HGI) or low glycaemic index (LGI) carbohydrate meal (preceding a 12 h overnight fast and where the meal was ingested 45-min prior to activity) on intermittent sprint and endurance exercise performance. Ten male varsity athletes from intermittent sports (age 23.6 ± 1.7 years, VO 51.9 ± 4.7 mL·kg·min) underwent a peak velocity (V) test and familiarisation session, followed by two experimental sessions in random order. Experimental sessions involved the ingestion of either an HGI or LGI meal, followed by the completion of the modified Loughborough Intermittent Shuttle Test (mLIST). There was no significant difference between HGI or LGI meals on sprint times ( = 0.62) and distance to exhaustion ( = 0.54) in the mLIST. Exercise heart rate, blood lactate and ratings of perceived exertion were also similar between the two meal trials throughout the mLIST (all > 0.05). Subjective ratings of hunger, fullness, satiety and satisfaction were also not significantly different between the two meals. In conclusion, consuming either an HGI or LGI meal after a prolonged 12 h fast and ingesting the meal 45 min prior to exercise did not differ in either physiological, subjective and intermittent sprint and endurance performance outcomes.