Effects of fructans and probiotics on the inhibition of Klebsiella oxytoca and the production of short-chain fatty acids assessed by NMR spectroscopy.
Abstract
Generally, the selection of fructans prebiotics and probiotics for the formulation of a symbiotic has been based on arbitrary considerations and in vitro tests that fail to take into account competitiveness and other interactions with autochthonous members of the intestinal microbiota. However, such analyzes may be a valuable step in the development of the symbiotic. The present study, therefore, aims to investigate the effect of lactobacilli strains and fructans (prebiotic compounds) on the growth of the intestinal competitor Klebsiella oxytoca, and to assess the correlation with short-chain fatty acids production. The short-chain fatty acids formed in the fermentation of the probiotic/prebiotic combination were investigated using NMR spectroscopy, and the inhibitory activities were assessed by agar diffusion and co-culture methods. The results showed that Lactobacillus strains can inhibit K. oxytoca, and that this antagonism is influenced by the fructans source and probably associated with organic acid production.
Keywords
lactic acid
co-culture
acetic acid
symbiotic
agar (pubchem cid: 76645041)
ammonium citrate (pubchem cid: 6435836)
calcium carbonate (pubchem cid: 10112)
dl-mandelic acid (pubchem cid: 1292)
deuterium oxide (pubchem cid: 24602)
dipotassium hydrogen phosphate (pubchem cid: 24450)
ethanol (pubchem cid: 702)
glucose (pubchem cid: 5793)
glycerol (pubchem cid: 753)
inulin from chicory (pubchem cid:16219508)
klebsiella oxytoca
l-cysteine hydrochloride (pubchem cid:60960)
lactobacillus acidophilus
magnesium sulfate (pubchem cid: 24083)
manganese sulfate (pubchem cid: 24580)
sodium acetate (pubchem cid: 517045)
sodium chloride (pubchem ci: 5234)
sodium hydroxyl (pubchem cid: 14798)
sucrose (pubchem cid: 5988)
sulfuric acid (pubchem cid: 1118)
tween 80 (pubchem cid: 86289060)
Access
Citation
ID:
121419
References
Blockchain Verification
Account:
NFT Contract Address:
0x95644003c57E6F55A65596E3D9Eac6813e3566dA
Article ID:
121419
Unique Identifier:
S0144-8617(20)31005-5
Network:
Scimatic Chain (ID: 481)
Loading...
Blockchain Readiness Checklist
Authors
Abstract
Journal Name
Year
Title
Creates 1,000,000 NFT tokens for this article
Token Features:
- ERC-1155 Standard NFT
- 1 Million Supply per Article
- Transferable via MetaMask
- Permanent Blockchain Record
Scan with Saymatik Web3.0 Wallet
