Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples
Abstract
Three 16S rRNA hybridization probes were developed and tested for genus-specific detection of Bifidobacterium species in the human fecal flora. Variable regions V2, V4, and V8 of the 16S rRNA contained sequences unique to this genus and proved applicable as target sites for oligodeoxynucleotide prob …
Keywords
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
RNA
humans
pubmed abstract
nih
national institutes of health
national library of medicine
DNA
Ribosomal
Base Sequence
Molecular Sequence Data
feces / microbiology
16s / genetics*
in situ hybridization
species specificity
bifidobacterium / classification
bifidobacterium / isolation & purification
bacterial / genetics
16s / isolation & purification
bacterial / isolation & purification
bacterial / genetics*
fluorescence / methods*
pmid:7487040
pmc167584
doi:10.1128/aem.61.8.3069-3075.1995
p s langendijk
f schut
g w welling
bifidobacterium / genetics*
dna probes / genetics
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119658
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