Autolysin-targeted LightCycler assay including internal process control for detection of Streptococcus pneumoniae DNA in clinical samples
Abstract
The development and clinical evaluation of a LightCycler PCR assay, including an internal process control (IPC), to detect the Streptococcus pneumoniae autolysin gene in clinical samples is reported. The assay was developed to provide a second target for use in conjunction with existing pneumolysin …
Keywords
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
humans
pubmed abstract
nih
national institutes of health
national library of medicine
DNA
sensitivity and specificity
Bacterial / analysis
Polymerase Chain Reaction / methods
lung / microbiology
pneumococcal infections / diagnosis*
bacterial / blood*
bacterial / cerebrospinal fluid
pneumococcal infections / microbiology
streptococcus pneumoniae / genetics*
streptococcus pneumoniae / isolation & purification
sputum / microbiology
pmid:14970243
doi:10.1099/jmm.0.05460-0
carmen l sheppard
timothy g harrison
robert c george
n-acetylmuramoyl-l-alanine amidase / genetics*
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115084
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