Amplification of cDNA ends based on template-switching effect and step-out PCR.
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Amplification of cDNA ends based on template-switching effect and step-out PCR.

M Matz, D Shagin, E Bogdanova, O Britanova, S Lukyanov, L Diatchenko, A Chenchik;M Matz;D Shagin;E Bogdanova;O Britanova;S Lukyanov;L Diatchenko;A Chenchik;
Nucleic Acids Research 1999 Vol. 27 pp. 1558-
102
chenchik1999nucleicamplification

Abstract

A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. ...

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DOI:
10.1093/nar/27.6.1558
URL:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC148354...

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ID: 112938
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112938
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10.1093/nar/27.6.1558
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