Concrete compressive strength is an important parameter of material properties for assessing seismic performance of reinforced concrete (RC) structures, which has a certain level of uncertainty due to its inherent variability. In this paper, the method of concrete strength validation of finite element model using smart aggregate (SA)-based stress monitoring is proposed. The FE model was established using Open System for Earthquake Engineering Simulation (OpenSEES) platform. The concrete strengths obtained from the material test, peak stress of SA, and estimated concrete strength based on SA stress were employed in FE models. The lateral displacement monitored by Liner variable differential transformer and vertical axial load monitored by load cell in the experiment are applied in the model. By comparing the global response (i.e., lateral reaction force and hysteretic loop), local response (i.e., concrete stress, rebar strain, and cross-section moment) and corresponding root-mean-square error obtained from experiment and numerical analysis, the capabilities of validation of FE model using SA-based stress monitoring method were demonstrated.