Evaluation and improvement of real-time PCR assays targeting lytA, ply, and psaA genes for detection of pneumococcal DNA
Abstract
The accurate diagnosis of pneumococcal disease has frequently been hampered not only by the difficulties in obtaining isolates of the organism from patient specimens but also by the misidentification of pneumococcus-like viridans group streptococci (P-LVS) as Streptococcus pneumoniae. This is especi …
Keywords
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
humans
pubmed abstract
nih
national institutes of health
national library of medicine
research support
non-u.s. gov't
u.s. gov't
non-p.h.s.
Child
DNA
Genes
sensitivity and specificity
Evaluation Study
Polymerase Chain Reaction / methods*
bacterial*
cerebrospinal fluid / microbiology
streptococcus pneumoniae / genetics
streptococcus pneumoniae / isolation & purification*
false positive reactions
pneumococcal infections / diagnosis
bacterial / analysis*
bacterial / genetics
nucleic acid hybridization
pmid:17537936
pmc1951257
doi:10.1128/jcm.02498-06
maria da gloria s carvalho
maria lucia tondella
jacquelyn s sampson
otitis media with effusion / microbiology
pneumococcal infections / microbiology*
serum / microbiology
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112251
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