Diagnosis of bacteremic pneumococcal pneumonia by amplification of pneumolysin gene fragment in serum
Abstract
A polymerase chain reaction (PCR) assay based on the amplification of pneumolysin gene fragments in patient sera was developed for the etiologic diagnosis of acute pneumococcal pneumonia. Two pairs of oligonucleotide primers were designed to amplify a 348-bp and a 208-bp fragment of the pneumolysin …
Keywords
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
humans
pubmed abstract
nih
national institutes of health
national library of medicine
research support
non-u.s. gov't
adult
aged
Genes
sensitivity and specificity
Polymerase Chain Reaction / methods*
bacterial*
pneumonia
pneumococcal / diagnosis*
streptococcus pneumoniae / genetics
bacteremia / diagnosis*
bacterial proteins
pmid:7844395
doi:10.1093/infdis/171.2.479
p salo
a ortqvist
m leinonen
streptolysins / genetics*
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112139
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