Retroviral expression in embryonic stem cells and hematopoietic stem cells

Retroviral expression in embryonic stem cells and hematopoietic stem cells

Cherry SR;Biniszkiewicz D;van Parijs L;Baltimore D;Jaenisch R;;
molecular and cellular biology 2000 Vol. 20 pp. -
360
sr2000molecularretroviral

Abstract

Achieving long-term retroviral expression in primary cells has been problematic. De novo DNA methylation of infecting proviruses has been proposed as a major cause of this transcriptional repression. Here we report the development of a mouse stem cell virus (MSCV) long terminal repeat-based retrovir …

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