Amplification of cDNA ends based on template-switching effect and step-out PCR

Amplification of cDNA ends based on template-switching effect and step-out PCR

Matz, M.;Shagin, D.;Bogdanova, E.;Britanova, O.;Lukyanov, S.;Diatchenko, L.;Chenchik, A.;
Nucleic Acids Research 1999 Vol. 27 pp. 1558-1560
405
m.1999nucleicamplification

Abstract

Abstract. A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product wit

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