Since carbon nanotubes and conjugated polymers promise to lead a number of real-world technologies, herein, an electrochemical biosensor, using poly[(9,9-dioctylfluorenyl-2,7-diyl)-alt-co-(1,4-benzo-{2,1’,3}-thiadiazole)] (PFBTz) and multi-walled carbon nanotubes (MWCNTs) was constructed for glucose sensing. To create such a surface, a graphite electrode was covered with PFBTz and MWCNTs, respectively. Then, glucose oxidase (GOx) was immobilized onto this new platform using glutaraldehyde (GA) as the cross linker. The limit of detection, linear range and sensitivity values were found to be 0.018 mM, 0.025–0.6 mM and 66.0 μAmM -1 cm -2 , respectively. The selectivity of the prepared biosensor was also examined in the presence of interferences and as a result, it has been observed that the biosensor response was much higher for glucose in all measurements. Surface characterizations before and after enzyme immobilization were done using scanning electron microscopy (SEM) and cyclic voltammetry (CV) techniques. In the final part of the study, the constructed biosensor was applied to beverages for glucose detection and very promising results were obtained. With this work, a robust, novel and economic sensing platform was prepared for glucose determination.