This study was aimed to investigate antioxidant, anticholinesterase inhibitory and tyrosinase inhibitory activities of n-Hex, DCM, EtOAc, and n-BuOH extracts obtained from rhizome and stem of Iris xanthospuria. Generally, it exhibited the best activity in both antioxidant activity tests of the rhizome and stem EtOAc extracts of the plant. EtOAc extracts reflected moderate activity on butyrylcholinesterase enzyme in BChE assay. EtOAc extract of rhizome showed the good tyrosinase inhibitory activity IC50 value of 4.30±0.25 g/L. In this study, EtOAc extracts may be potentially a source of natural antioxidant, anticholinesterase inhibitory and tyrosinase inhibitory activities.