The algal species were collected from the siren river Hazara division and were isolated and identified. Various species were selected to optimize the production of different biofuels. In order to efficiently produce biodiesel from algae, strains have to be selected with a high growth rate and oil content. If an open culture system was used, then the selected strain must have the ability to remain dominant under the applied conditions. The main species identified wereTribonema, Ulothrix, Hydrodictyon reticulum, Bubble, Euglena, Oedogonium, and Spirogyra.Amongst these species Tribonema specie showed the highest oil content which could be used for bio-fuel in future. It was concluded from the experiment that the siren valley had various algal species and Tribonema sp. was best for its oil content, which could be used for future bio-fuel purposes.