This article reveals the qualitative analysis of different metabolites, seven heavy metals concentration and antioxidant activity of the fruit of Solanum nigrum (L). Qualitative analysis is very essential for identifying the various classes of compounds present in the medicinal plants. Solanum nigrum (L) is an important herbaceous medicinal plant belongs to Solanacae family. Phytochemical screening of the crude extracts revealed the presence of secondary compounds such as alkaloids, flavonoids, steroids and tainns. Also the concentration of seven heavy metals Zinc (Zn), Cobalt (Co), Lead (Pb), Nickle (Ni), Cadmium (Cd), Iron (Fe) and Copper (Cu) were determined. On comparison the concentration of all metals were high in Solanum nigram. The iron was in high amount 46.46 mg/L, while cobalt was in less amount 0.082 mg/L in Solanum nigram. The fruit extract show a good antioxidant activity at low concentration at 20 and 40 ppm.