Potential Antioxidant and Enzyme Inhibitory Effects of Nanoliposomal Formulation Prepared from Rech. f. Extract.

Potential Antioxidant and Enzyme Inhibitory Effects of Nanoliposomal Formulation Prepared from Rech. f. Extract.

Karatoprak, Gökçe Şeker;Yücel, Çiğdem;Göger, Fatih;Sobarzo-Sánchez, Eduardo;Küpeli Akkol, Esra;
Antioxidants (Basel, Switzerland) 2020 Vol. 9
281
karatoprak2020potentialantioxidants

Abstract

Rech. f. is a species that grows only in Hatay, Turkey and is used as a traditional stomachic tea. Neither the chemical composition nor the potential bioactivity of the plant has been investigated before. Antioxidant activity (1,1-Diphenyl-2-picrylhydrazyl Radical (DPPH) and 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging and β-carotene/linoleic acid co-oxidation) of 70% methanol, 70% ethanol extracts, and 2% infusion obtained from aerial parts were determined. The effect of 70% methanol extract on collagenase and elastase enzyme inhibition and its chemical composition via chromatographic methods (LC-MS/MS and HPLC) were analyzed. Nanoliposomes were developed with 70% methanol extract, were characterized, and were evaluated. The key parameters for the most active 70% methanol extract included the following DPPHEC: 28.4 µg/mL, Trolox equivalent antioxidant capacity (TEAC)/ABTS: 1.77 ± 0.09 mmol/L/Trolox. Furthermore 70% methanol extract showed more than 50% inhibition on collagenase and elastase enzymes at all the concentrations. The main component of the extract, rich in phenolic compounds, has been identified as rosmarinic acid; 83.7 µg/mL extract was released from the nanoliposomal formulation. The extract and its formulation are found to be nontoxic on the L929 fibroblast cell line. This study successfully developed a long-term antioxidant and enzyme inhibitory formulation containing , which has been used safely among the public for years.

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