The increasing resistance of bacteria to antibiotics has motivated the interest in potent natural compounds capable of disrupting bacterial cell-to-cell communication. Column chromatography of seed extract of Annona senegalensis afforded N-cerotoyltryptamine (1), asimicin (2) and ent-19-carbomethoxykauran-17-oic acid (3). The compounds were tested for their antimicrobial, antibiofilm, and anti-quorum sensing activities. The minimum inhibitory concentrations (MIC) values ranged from 0.5 μg/mL to 1 μg/mL for C. albicans ATCC 10239 and S. aureus ATCC 25923 E. coli ATCC 25922, C. violaceum CV026 and C. violaceum CV12472. Compound 2 exhibited the best activity (MIC of 0.5 μg/mL) on all tested bacterial strains except on S. aureus ATCC 25923. All the compounds inhibited biofilm formations of all microorganisms tested in various percentages at MIC and MIC/2. Compound 2 also exhibited the highest antibiofilm activity against C. albicans (yeast) and E. coli with percentage inhibitions ranging from 6.3±4.1 (MIC/4) to 37.9±4.5 (MIC) for C. albicans and from 18.8±1.1 (MIC/4) to 43.2±0.5 (MIC) for E. coli. Compound 1, however, showed highest biofilm inhibition on S. aureus as the percentage inhibition varied from 26.7±3.6 (MIC/4) to 43.8±2.1 (MIC). Compound 2 showed highest percentage violacein inhibition on C. violaceum CV12472 ranging from 10.2±0.5 (MIC/8), 65.76±1.3 (MIC/2) and 100 (MIC). Compound 1 and 3 had percentage violacein formation inhibitions on C. violaceum CV12472 ranging from 9.66±1.1 (MIC/4) to 100 (MIC), and from 17.4±2.4 (MIC/4) to 100 (MIC), respectively. Swimming and swarming motility of P. aeruginosa PA01 strain was evaluated at three concentrations of 50, 75 and 100 μg/mL. The compounds inhibited the P. aeruginosa swimming and swarming motility at the three tested concentrations (50, 75 and 100 μg/ml) in a dose-dependent manner. The extents of inhibition of motility migration was relatively higher in the swimming model than in the swarming model for all compounds. Compound 1 exhibited the highest percentage inhibition of motility of 41.50±3.5 and 39.73±1.5 in swimming model and swarming model respectively at 100 μg/ml. Compound 3 showed the lowest percentage inhibition of 30.36±2.0 and 23.50±2.5 in swimming and swarming respectively at 100 μg/ml. At the lowest tested concentration of 50 μg/ml, it was compound 2 showing the highest inhibition of motility of 17.49±0.5 and 14.29±1.0 in swimming and swarming respectively. Compound 1 showed highest quorum sensing (QS) activity with QS inhibition zone of 20.0±1.5 mm at MIC and 11.0±1.0 mm at MIC/8 while compound 2 had the highest antimicrobial (AM) zone diameter amongst the compounds at MIC. Compound 3 was the QS inhibitory sample and did not show any QS inhibition at MIC/8 while showing its highest QS inhibition zone of 13.0±1.6 mm at MIC. For antioxidant assays, no sample showed better activity than the standards. Compound 2 had highest activity with IC values of 87.79±2.70 and 42.77±1.53 μg/mL in DPPH and β-carotene-linoleic acid assay respectively and was more active (IC of 97.69±1.40 μg/mL) than standard quercetin (IC 250.09±0.87 μg/mL) in metal chelation assay.