Physician assistants (PAs) are a growing group of healthcare providers who could facilitate the adoption of electronic healthcare (e-Health) into practice. In 2018, we conducted a Canada-wide web-based survey study of practicing PAs and student PAs regarding their current exposure to e-Health, as well as their perceived value for its use and interest in future adoption. For this study, e-Health was defined as technology that allows direct communication between patients and healthcare providers or facilitates patient self-management for the purpose of assessment and management. We focused on telehealth, direct messaging (e.g. text, email), patient-directed web-based applications (apps) and patient-provider shared web-based apps. Survey responses were analysed descriptively and compared between practicing and student PAs with Chi-square tests of independence. We also examined correlations between age, exposure, perceived value and interest in future adoption for practicing PAs and student PAs separately. About 186 respondents completed the survey; 145 practicing PAs and 39 student PAs. Fewer than half of respondents had exposure to the studied e-Health modalities. Compared to practicing PAs, student PAs more often perceived value in e-Health and expressed interest in its expanded adoption. In both groups, perceived value frequently correlated significantly with interest in adoption. Student PAs report little formal education during their training, and both practicing PAs and student PAs note a need for infrastructure support, and general knowledge about what is available and safe in order to enable them to expand their use of e-Health in practice. The most interest is present for patient-directed apps and patient-provider shared apps. With workload and remuneration barriers to physician adoption of e-Health, salary-based PAs could have a role in facilitating the integration of e-Health solutions into practice. Additional awareness, exposure and support for PAs to do so are required.