Applications of antioxidants are increasing due to their multiple roles in removing harmful effects of oxidative stress. For the present study TPC, DPPH, FRAP, Metal Chelating Ability, Reducing Power and TAC spectrophotometric assays were used for the assessment of antioxidative  properties of methanolic extracts of leaves of 5 different Cleome species (Cleome viscosa L., C. simplicifolia (Camb.) Hook f. & Thoms, C. gynandra L., C.  chelidonii L.f. and C.  speciosa Raf.). The purpose of this study was also to evaluate the comparability of antioxidant capacity measurements in Cleome species by the above assay. This study may provide some supplementary food option by some Cleome species.