Optimal conditions for protein synthesis in incubated slices of rat brain
Abstract
Optimal conditions for protein synthesis in incubated slices of rat brain were determined to be: thickness 0.3 mm under air (for newborn 0.4-0.6 mm) or 0.5 mm under oxygen; temperature, 35-36 degrees C; hepes (N-2-hydroxyethylpiperazine-N'-2-ethane sulfonic acid) buffer; K+, 6-8 mM; Ca2+, 2-3 mM. Th …
Keywords
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
Depression
animals
pubmed abstract
nih
national institutes of health
national library of medicine
research support
u.s. gov't
male
Comparative Study
P.H.S.
Rats
Temperature
in vitro techniques
kinetics
hydrogen-ion concentration
amino acids / metabolism
brain / metabolism*
chemical
buffers
calcium / pharmacology
magnesium / pharmacology
potassium / pharmacology
pmid:241463
doi:10.1016/0006-8993(75)90031-1
d s dunlop
w van elden
a lajtha
nerve tissue proteins / biosynthesis*
ouabain / pharmacology
sodium / pharmacology
stimulation
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270232
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