Alterations in the relative amounts of specific mRNA species in the developing human brain in Down's syndrome
Abstract
Total cellular polyadenylated RNA [poly(A)+ RNA] was prepared after guanidinium thiocyanate extraction of frozen brain tissue from age-matched normal and Down's-syndrome (trisomy 21) human foetuses. Poly(A)+ RNA populations were analysed by translation in vitro, followed by two-dimensional gel analy …
Keywords
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
RNA
humans
pubmed abstract
nih
national institutes of health
national library of medicine
research support
non-u.s. gov't
protein biosynthesis
electrophoresis
brain / metabolism*
messenger / metabolism*
centrifugation
polyacrylamide gel
microtubules / metabolism
pmid:6204644
pmc1153608
doi:10.1042/bj2200179
s a whatley
c hall
l lim
brain / embryology
density gradient
down syndrome / embryology
down syndrome / metabolism*
endopeptidases
fetal proteins / metabolism
photofluorography
poly a / metabolism
rna / metabolism
serine endopeptidases*
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266679
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