Acetylcholinesterase is one of the most important enzymes in living organisms, which is responsible for the synapse cholinergic and other nervous processes. However, its inhibiting effects have proven to have pharmacological applications in the treatment of different diseases, as well as in the control of insect pests; thus, the search for inhibitors is a matter of interest for biomedical and agrochemical fields. Alzheimer’s is a progressive neurodegenerative disease, which can be seen as a wide degeneration of synapses, as well as neurons, in the cerebral cortex, hippocampus, and subcortical structures. Acetylcholinesterase inhibition is an important target for the management of Alzheimer’s. Additionally, diabetes mellitus is a chronic disease with clinical manifestation of hyperglycemia, due to the ineffective production of insulin that controls the level of blood glucose. Alphaglucosidaseinhibitors could retard the uptake of dietary carbohydrates and have shown significant therapeutic effects in clinical application. Fifty-five ethyl acetate extracts from nine bacterial families from Hainan (China) were evaluated to observe their acetylcholinesterase, alphaglucosidase, and xanthine oxidase inhibitory activity. Moreover, a screening of inhibitory activity against the pathogens fungi Fusarium oxysporum and Colletotrichum gloeosporioides was performed. The best acetylcholinesterase and alphaglucosidase inhibitory activity was shown by Vibrio neocaledonicus (98.95%). This is the first report of inhibition of both enzymes by ethyl acetate extract from this strain.