Abstract   Background & aim: Exposure to electromagnetic waves can lead to oxidative stress. On the other hand, the antioxidant system function of the body increases with regular exercise and reduces oxidative stress. The aim of this study was to investigate the effect of eight weeks aerobic training on oxidative stress markers in rats exposed to electromagnetic microwave radiation emitted from the Wi-Fi routers.   Methods: In this experimental study, 26 male Wistar rats (8 weeks old) with a weight range of 200±20 g were randomly divided into four groups including: control, radiation, exercise, and radiation + training. The main training program consisted of 8 weeks (one session a day and five days a week) running on a treadmill for animals at 40 to 60% max speed, and the protocol included one hour of exposure to a 45.2 GHz rays of Wi-Fi modem for 8 weeks. Before and 48 hours after the main interventions, blood samples were taken from all groups. Plasma superoxide dismutase (SOD) and glutathione peroxidase (GPX) levels were measured by ELISA and validated by MDA and MDA kits with TBA method. Data was analyzed using one way ANOVA, Tukey and T-test.   Results: The results of one-way ANOVA showed that there was a significant difference among the different groups in the study. Eight weeks of exposure to Wi-Fi radiation caused a decrease in plasma levels of SOD and GPx and an increase in MDA. On the other hand, eight weeks of aerobic exercise increased plasma levels SOD and GPx, and reduced MDA. Tukey's test showed that there was a significant difference between SOD and GPx in all groups, except for those with radiation + (p = 0.099 and p = 0.073). In MDA, the difference was significant among all groups, except for the aerobic group + aerobic training group with aerobic training and control groups (p = 0.331 and p = 0.717). The results of t-test showed a significant difference between pre-test and post-test of all variables in the research.   Conclusion: It seems that regular aerobic exercise exercises can have beneficial effects on the antioxidant system and modulate the oxidative stress indices induced by the Wi-Fi radiation.