Scientific understanding of aerosol-cloud interactions can profit from an analysis of the transition regions between pure aerosol and pure clouds as detected in satellite data. This study identifies and evaluates pixels in this region by analysing the residual areas of aerosol and cloud products from the Moderate Resolution Imaging Radiometer (MODIS) satellite sensor. These pixels are expected to represent the “twilight zone” or transition zone between aerosols and clouds. In the analysis period (February and August, 2007–2011), about 20% of all pixels are discarded by both MODIS aerosol and cloud retrievals (“Lost Pixels”). The reflective properties and spatial distribution of Lost Pixels are predominantly in between pure aerosol and cloud. The high amount of discarded pixels underlines the relevance of analyzing the transition zone as a relevant part of the Earth’s radiation budget and the importance of considering them in research on aerosol-cloud interactions.