Comparison of real-time PCR with SYBR Green I or 5'-nuclease assays and dot-blot hybridization with rDNA-targeted oligonucleotide probes in quantification of selected faecal bacteria
Abstract
PCR primers and hybridization probes were designed for the 16S rRNA genes of six bacterial species or groups typically present in human faeces or used in the dairy industry. The primers and probes were applied for quantification of the target bacterial genomes added in artificial DNA mixtures or fae …
Keywords
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
RNA
humans
pubmed abstract
nih
national institutes of health
national library of medicine
research support
non-u.s. gov't
Comparative Study
DNA
Ribosomal
16S / genetics
pmid:12576600
doi:10.1099/mic.0.25975-0
Evaluation Study
Erja Malinen
Anna Kassinen
Airi Palva
Bacteria / genetics
Bacteria / isolation & purification*
Bacterial / analysis
Ribosomal / analysis
Feces / microbiology*
Fluorescent Dyes / metabolism
Immunoblotting
Nucleic Acid Hybridization / methods*
Oligonucleotide Probes*
Organic Chemicals*
Polymerase Chain Reaction / methods*
Taq Polymerase / metabolism
Citation
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110520
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