Recent studies have shown that cattle practitioners are concerned about painful conditions and procedures. An understanding of the attitudes toward pain is fundamental to encourage the use of pain relief in cattle. The goal of this study was to investigate the factors that influence Brazilian professionals toward the use of pain mitigation in cattle, primarily during castration and horn removal (e.g. dehorning, disbudding). To reach this objective, an online survey was conducted with veterinarians and animal scientists. The analysis was based on descriptive statistics, chi-squares tests, Fisher exact tests, and Mann-Whitney U tests. The final sample was composed of 147 respondents. Results of descriptive statistics showed that the use of medicine before a painful procedure (i.e., anesthetic, anti-inflammatory, or sedative-analgesic), was higher for adult cattle than for suckled and newborn calves. In newborn calves, those respondents who were more likely to use pain relief agreed with the statements that i) there is missing information about pain control during castration and ii) defensive movements shown by an animal during or after a procedure to justify pain mitigation in horn removal. The primary reasons for choosing a medicine were similar for both procedures: cost, anti-inflammatory effect, analgesic potency, duration of analgesic effect, duration of sedative effect, and route of administration. There was no difference in professional's decade of birth and pain mitigation use. These results may be useful to decide how to optimize the use of drugs by veterinary and animal science professions, and continuing education should help to increase pain mitigation usage.