One of the alternatives to antimicrobials in disease control is probiotic bacteria as microbial control agents. This research was carried out to obtain indegenous isolates of probiotic bacteria from digestive tract Cyprinus carpio. Selection criteria were appointed based on the capacity to produce the antibacterial compound against the pathogen and grow at pH 2. The research was conducted from May to July 2014 at Microbiology Laboratory, Biology Department, UNSYIAH. The research was carried out by experiment laboratories. The planting of the bacteria was carried out with the pour-plate method in nutrient agar medium. The result show that there are six bacterial isolates varied morphological colony and cell and able to produce antibacterial compounds for active against A.hydrophila and E. tarda.