Digital Elevation Model (DEM) is one of the important parameters for soil erosion assessment. Notable uncertainties are observed in this study while using three high resolution open source DEMs. The Revised Universal Soil Loss Equation (RUSLE) model has been applied to analysis the assessment of soil erosion uncertainty using open source DEMs (SRTM, ASTER and CARTOSAT) and their increasing grid space (pixel size) from the actual. The study area is a part of the Narmada river basin in Madhya Pradesh state, which is located in the central part of India and the area covered 20,558 km2. The actual resolution of DEMs is 30 m and their increasing grid spaces are taken as 90, 150, 210, 270 and 330 m for this study. Vertical accuracy of DEMs has been assessed using actual heights of the sample points that have been taken considering planimetric survey based map (toposheet). Elevations of DEMs are converted to the same vertical datum from WGS 84 to MSL (Mean Sea Level), before the accuracy assessment and modelling. Results indicate that the accuracy of the SRTM DEM with the RMSE of 13.31, 14.51, and 18.19 m in 30, 150 and 330 m resolution respectively, is better than the ASTER and the CARTOSAT DEMs. When the grid space of the DEMs increases, the accuracy of the elevation and calculated soil erosion decreases. This study presents a potential uncertainty introduced by open source high resolution DEMs in the accuracy of the soil erosion assessment models. The research provides an analysis of errors in selecting DEMs using the original and increased grid space for soil erosion modelling.