Campylobacter (C.) jejuni from persons suffering from diarrhoea, from organs of poultry, C. jejuni and C. fetus ssp. fetus from the gastrointestinal tract of calves and adult cattle as well as a number of reference strains were examined for cytotoxin formation in a CHO-K1 cell culture test. During evaluation, three morphologically different pictures were observed. The first cytotoxin caused a formation of strikingly large, rounded or polymorphic and elongated cells which was associated with reduced growth. The progressive morphological changes corresponded to those described for the Cytolethal Distending Toxin (CLDT) and were assigned to it. The second cytotoxin produced a rounding of cells without a change in their size while at the same time, growth was reduced. In analogy to CLDT, this toxin was termed Cytolethal Rounding Toxin (CLRT). A third morphological picture consisted of cell changes characterized by enlarged polymorphic as well as by small rounded cells. These cell changes were considered as being distinct from the above mentioned ones and referred to as CLTD/CLRT effect. In none of the 39 Campylobacter strains isolated from humans and calves with diarrhoea, a noteworthy cytotonic activity could be detected that would indicate the presence of an enterotoxin.