In this study, we evaluate whether diffusion-weighted magnetic resonance imaging (DW-MRI) data after denoising can provide a reliable estimation of brain intravoxel incoherent motion (IVIM) perfusion parameters. Brain DW-MRI was performed in five healthy volunteers on a 3 T clinical scanner with 12 different b-values ranging from 0 to 1000 s/mm . DW-MRI data denoised using the proposed method were fitted with a biexponential model to extract perfusion fraction (PF), diffusion coefficient (D) and pseudo-diffusion coefficient (D*). To further evaluate the accuracy and precision of parameter estimation, IVIM parametric images obtained from one volunteer were used to resimulate the DW-MRI data using the biexponential model with the same b-values. Rician noise was added to generate DW-MRI data with various signal-to-noise ratio (SNR) levels. The experimental results showed that the denoised DW-MRI data yielded precise estimates for all IVIM parameters. We also found that IVIM parameters were significantly different between gray matter and white matter (P < 0.05), except for D* (P = 0.6). Our simulation results show that the proposed image denoising method displays good performance in estimating IVIM parameters (both bias and coefficient of variation were <12% for PF, D and D*) in the presence of different levels of simulated Rician noise (SNR = 20-40). Simulations and experiments show that brain DW-MRI data after denoising can provide a reliable estimation of IVIM parameters.