We report the emergence in Kenya during 1997-1999 of typhoid fever due to Salmonella enterica serovar Typhi resistant to ampicillin, tetracycline, chloramphenicol, streptomycin, and cotrimoxazole. Genotyping by pulsed-field gel electrophoresis of XbaI-digested chromosomal DNA yielded a single cluster. The multidrug-resistant S. Typhi were related to earlier drug-susceptible isolates but were unrelated to multidrug-resistant isolates from Asia.