A field experiment was conducted during 2014 and 2016 rainy season at Tudun Wada, Kano and Shika, Zaria in the Northern Guinea Savanna of Nigeria in order to study the physiological responses of maize hybrids under low nitrogen. The experiment consisted of two nitrogen levels 0 and 120 N kg ha−1 as main plot and 8 drought-tolerant maize hybrids and 2 controls as subplot laid out in a randomized split plot design and replicated three times. Physiological parameters of hybrids were significantly affected by low nitrogen at both locations. Interaction between hybrids and nitrogen was significantly affected at both locations. Based on these results, application of nitrogen significantly increased the physiological growth indices of maize hybrids. The extent of increment in physiological reactions was additionally higher in Zaria in view of higher soil natural carbon and nitrogen and higher precipitation was better dispersed at this area. However recent hybrids were more tolerant to nitrogen stress and out-yielded the older hybrids. Therefore the recently released hybrids were more adapted to abiotic stresses.