Essential oil composition of Gypsophila turcica Hamzaoğlu, Gypsophila pinifolia Boiss. & Hausskn., G. tuberculosa Hub.-Mor., G. eriocalyx Boiss. and G. laricina Schreb. were analyzed by means of gas chromatography-mass spectrometry (GC-MS). Thirty six, fourty four, sixty six, forty one and sixty one compounds were identified in the essential oils of G. turcica, G. pinifolia, G. laricina, G. tuberculosa and G. eriocalyx respectively. The major components were determined hentriacontane (12.93 ± 0.4%), 1-octadecanol (8.97 ± 0.1%), hexahydrofarnesyl acetone (6.9 ± 0.09%) and pentacosane (6.63 ± 0.08%) in G. turcica oil, hexadecanoic acid (17.6 ± 0.4%), 1-tetradecanol (7.6 ± 0.1%) and phytol (5.63 ± 0.05%) in G. pinifolia oil, octacosane (6.83%), eicosanal (6.19%), triacontane (6.03%) and heneicosane (5.78%) for G. eriocalyx, hexadecanoic acid (25.3%, 27.0%) and hentriacontane (13.0%, 12.6%) for G. tuberculosa and G. laricina, respectively. Antibacterial activity of G. eriocalyx, G. tuberculosis and G. laricina were investigated against Gram negative (Escherichia coli) and Gram positive (Staphylococcus aureus) bacteria.