In the present work, selective adsorption of β-glucosidase using imprinted polyacrylamide hydrogels were studied. For this purpose imprinted hydrogels were prepared using β-glucosidase as a template molecule, acrylamide (AAm) as a monomer, N,N’- methylenebisacrylamide (MBAA) as a crosslinker, ammonium persulphate (APS) and N,N,N’,N’-tetramethylethylene-diamine (TEMED) as initiators. β-Glucosidase imprinted hydrogel was washed with a solution of sodium dodecyl sulfate (SDS) and acetic acid to remove the template molecule. Non-imprinted hydrogel was also prepared without using β-glucosidase template. The adsorption and recognition performance of hydrogels towards β-glucosidase was discussed through adsorption isotherms and adsorption kinetics. In batch template rebinding experiments, imprinted hydrogels displayed quite high template binding capacity than non-imprinted hydrogels. The theoretical maximum adsorption capacity (Qmax) was determined by the Langmuir model, which turned out to be 7.2 mg/g  and 4.6 mg/g   for imprinted and non-imprinted hydrogels respectively. A pseudo-second-order model was suitable to interpret kinetic data.