Contamination of chromium signifies one of the major threats to soil system. Phytoremediation is a promising technique to reclaim metal-contaminated soil using plants which are capable to tolerate and accumulate heavy metals within in their tissues. The experiment reported in this article was carried out with six biofuel plant species, Cyamopsis tetragonoloba, Glycine max, Avena sativa, Abelmoschus esculentus, Sesamum indicum and Guizotia abyssinica, were subjected to eight Cr concentrations (0.5, 2.5, 5, 10, 25, 50, 75 and 100 mg kg soil) to investigate Cr toxicity, tolerance and accumulation. After 12 weeks of experiment, Cr phytotoxicity on morphological and biochemical parameters were evaluated. For six plant species, seed germination and most of growth parameters were significantly (p < 0.05) reduced under high Cr stress. Chlorophyll contents were also decreased with increased Cr concentrations. Accumulation of Cr was higher in roots than shoot in all studied plants. Significant Cr accumulation was in the order of C. tetragonoloba > A. sativa > A. esculentus > S. indicum > G. max > G. abyssinica. Bioconcentration factor, bioaccumulation coefficient, translocation factor and phytoremdiation ratio suggested that C. tetragonoloba, A. sativa and A. esculentus being more tolerant; having higher Cr accumulation and could be a high efficient plants for reclamation of Cr-contaminated soils.