In this study, 416 hospital staffs from different departments and profession were screened for Hepatitis C virus (HCV) antibody by Enzyme Immune Assay method. Two hundred fifty blood bank donors were tested as a control group. Anti HCV positivity were found in four of hospital staffs (0.9%) and in four of donors (1.6%). According to our results, generally we didn't find any data about transmission of hepatitis C virus from hospital but it is interesting that all of the positive cases were from intensive care units.