Garcinol, a well-known medicinal phytochemical, was extracted and isolated from the dried fruit rinds of . In this study, garcinol has successfully used to reduce silver ions to silver in order to synthesize G-AgNPs. The formation and the structure of G-AgNPs were confirmed by UV-Visible spectroscopy, FTIR spectroscopy and TEM. The antimicrobial activity of garcinol and G-AgNPs were investigated by well diffusion assay, broth micro dilution assay and time-kill kinetic study, over selected five microbial species including (ATCC 25623), (ATCC 27853), (ATCC 25922), (ATCC 10231) and clinical isolates of Methicillin (MRSA). Antimicrobial activity of G-AgNPs was compared with the chemically synthesized silver nanoparticles (AgNPs). The formation of G-AgNPs is a promising novel approach to enhance the biologically activeness of silver nanoparticle and to increase the water solubility of garcinol which provides a significant array of therapeutic applications.