The Emotion Regulation Questionnaire (ERQ) measures habitual use of two emotion regulation strategies, cognitive reappraisal and expressive suppression. The present study tested the psychometric properties and validity of a newly developed Croatian adaptation of the ERQ. In a sample of 347 participants, we first sought to confirm the ERQ's original factor structure. Next, we tested its convergent, divergent and incremental validity. In the first subsample (N = 140), we linked the ERQ with a measure of the Five-Factor Model dimensions and their facets (Revised NEO Personality Inventory). In the second subsample (N = 207), we tested its ability to predict personality dimensions assessed by a different measure (Big Five Inventory) as well as the traits of positive and negative affect, including its incremental validity in predicting the latter. The results corroborated earlier findings, showing that the Croatian ERQ has equivalent psychometrics and predictive validity as ERQs in other languages. Crucially, the observed links between the ERQ and the facets of the Five-Factor Model dimensions enrich our understanding of the personality mechanisms behind the employment of these emotion regulation strategies.