The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications
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The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications

Götzke, Hansjörg;Kilisch, Markus;Martínez-Carranza, Markel;Sograte-Idrissi, Shama;Rajavel, Abirami;Schlichthaerle, Thomas;Engels, Niklas;Jungmann, Ralf;Stenmark, Pål;Opazo, Felipe;Frey, Steffen;
Nature communications 2019 Vol. 10 pp. 1-12
174
gotzke2019thenature

Abstract

Epitope tags are widely used in various applications, but often lack versatility. Here, the authors introduce a small, alpha helical tag, which is recognized by a high affinity nanobody and can be used in a range of different applications, from protein purification to super-resolution imaging and in vivo detection of proteins.

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