Dental caries among children is one of the greatest challenges faced by dentists globally; especially that of susceptible surfaces like the Pit and fissures. Dental sealants have proved to be an effective way to prevent caries development. The Clinical success of any material depends upon its adhesion to tooth structure, resistance to wear and ability to withstand the masticatory or occlusal forces. Hence it is important to evaluate the shear bond strength (SBS). The Present study's aim was to evaluate and compare the shear bond strength of different pit and fissure sealants placed on Primary molars and Permanent Premolars.Sixty noncarious extracted teeth comprising of thirty Primary molars and thirty Permanent Premolars were divided into four groups of 15 each. The buccal surfaces of all teeth were dried, etched and the etched surfaces of Primary molars (Group I) and Permanent Premolars (Group III) were placed with Helioseal-F while Groups II and IV, that included Primary molars and Permanent Premolars received Clinpro. Shear bond strength was evaluated and the mean was obtained for all the groups. The results were analyzed using twoway analysis of variance followed by Tukeys post hoc procedure to check for significant differences.The specimens of unfilled sealant Clinpro (Groups II & IV) showed higher Shear bond strength when compared to the specimens of filled sealant Helioseal-F (Groups I & III).The unfilled sealant showed a better Shear bond strength compared to the filled sealant. The bond strength in Primary molars was slightly higher compared to Permanent Premolars. How to cite the article: Pushpalatha HM, Ravichandra KS, Srikanth K, Divya G, Done V, Krishna KB, Patil V. Comparative evaluation of Shear bond strength of different Pit and fissure Sealants in Primary and Permanent teeth - An In-Vitro Study. J Int Oral Health 2014;6(2):84-9.