DDT is a hydrophobic organic pollutant, which can be bio-accumulated in nature and have adverse consequences on the physical condition of humans and animals. This study investigated the relationship between the white-rot fungus and biosurfactant-producing bacteria associated with the degradation of DDT. The effects of on fungal development were examined using confrontational assay on a Potato Dextrose Agar (PDA) medium. was added to the culture at 1, 3, 5, 7 and 10 mL (1 mL ≈ 1.44 × 10 CFU). After 7 d incubation, about 43% of the initial DDT (0.25 μmol) was degraded by the culture only. The augmentation of 7 mL of revealed the most superior's synergism with DDT degradation being approximately 78% and the ratio of optimization 1.06. According to the confrontational assay, promoted the growth of towards the bacterial colony, with no direct contact amid the bacterial cells and mycelium (0.71 cm/day). DDD (1,1-dichloro-2,2-bis(4-chlorophenyl) ethane), DDE (1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene), and DDMU (1,1-chloro-2,2-bis(4-chlorophenyl) ethylene) were identified as metabolite products, indicating that the could enhance the DDT biodegradation by .