Dyera lowii is an endemic and vulnerable tree species of commercial value as chewing gum found in peat swamp forests, scatteredly distributed in Sumatra, Kalimantan, and Peninsular Malaysia. Their existence is now under severe threat due to habitat conversion. This study is aimed to assess genetic diversity within four natural populations (Hampangen, Parahangan, Sebangau, Selat Nusa ) and one plantation in Central Kalimantan based on allozyme variation. Electrophoresis procedures were conducted with an isoelectric focusing polyacrylamide slab gel system. The result showed high genetic diversity (