The subject of this research was to evaluate the effect of the time of establishment (0-5 years, 5-10 years, and more than 10 years.) of pastures of Brachiaria sp. over theArbuscular Mycorrhizal Fungal populations (AMF): genus and spore distribution, length of extramatrical mycelia, root colonizations by AMF and septate fungi; the pastures resulting from fragmentation of the tropical rainforest with valley and hilly landscapes at Florencia, Caquetá, Colombia. Whichever the age of pasture, the genera Glomus and Acaulospora predominate; the change of coverage require more than ten years for different species of MAF to recolonize, adapt and diversify. In hilly terrain, the Brachiaria sp-MAF relationship decrease with time, specially after ten years of establishment; in valley landscapes the relationship is medium and homogeneous (21- 50%), while the root colonization by septate fungi increased, showing possibly a competitive mechanism by root space or a cooperative activity. Finally, the extramatrical mycelia and spores in any landscape had a variable behavior (increase, decrease or stability) with time.