Antibiotic resistance pollution is globalizing. However, little quantitative data exists regarding the occurrence of antibiotic resistance genes (ARGs) in Antarctica, which is central to assessing the level of global contamination of ARGs. Soil, sediment, and animal feces were sampled from 12 sites in the Fildes Peninsula in January and February 2017. The occurrence and distribution of qnrS, sul1 and sul2 were investigated. The results showed that sul1 was the predominant ARG and that all the ARGs were detected in animal feces. The total absolute abundance of the ARGs in animal feces (5.86 × 10 copies) was the highest. Strong and positive correlations between sul1 and int1 were found, indicating that int1 might play an important role in the creation and spread of ARGs. This data and the analysis are critical for filling the data gap regarding ARGs in Antarctica and for improving understanding of the globalization of antibiotic resistance pollution.